POTENTIAL INTERFERENCE OF HYDROGEN-PEROXIDE IN THE 2,2'-BICINCHONINIC ACID PROTEIN ASSAY

被引:12
作者
BAKER, WL
机构
[1] Chemistry Department, Swinburne Institute of Technology, Hawthorn, Vic. 3122, John Street
关键词
D O I
10.1016/0003-2697(91)90209-C
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
At a concentration of 20-800 nmol 0.1 ml hydrogen peroxide instantly reacts with a 2,2′-bicinchoninic acid copper color reagent. It also reacts with a reformulated reagent at pH 7 but the color develops less rapidly. While the effect may interfere with protein estimations at alkaline pH, the effect of pH 7 may be used to determine the possible extent of hydrogen peroxide interference after treatment with catalase. © 1991.
引用
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页码:212 / 214
页数:3
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