STUDIES ON ASPERGILLUS-FUMIGATUS ANTIGENS FOR SERODIAGNOSIS

被引：4

作者：

BANERJEE, B ^{[1
]}

MUKHERJEE, M ^{[1
]}

GANGAL, SV ^{[1
]}

SARMA, PU ^{[1
]}

机构：

[1] CSIR,CTR BIOCHEM,PATEL CHEST INST BLDG,UNIV CAMPUS,DELHI 110007,INDIA

来源：

SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE | 1990年 / 4卷 / 03期

关键词：

allergic bronchopulmonary aspergillosis; Aspergillus fumigatus antigens; ELISA; fast protein liquid chromatography;

D O I：

10.1016/0888-0786(90)90003-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Culture filtrate (CF) proteins of a soil strain of Aspergillus fumigatus were evaluated for their potential use in the serodiagnosis of patients of allergic bronchopulmonary aspergillosis (ABPA). Analytical fractionation of third-week culture filtrate (III WCF) and third-week mycelium (III W mat) by fast protein liquid chromatography (FPLC) indicated protein peaks in the molecular weight range of 180, 70, 43, 12 and 6·5 kDa. The III WCF proteins showed very strong precipitin reaction with the sera of ABPA patients by double immunodiffusion. The increased serum IgG and IgE levels (estimated by microtitre plate ELISA method) correlated well with the severity of the disease. The patients with strong precipitin reaction showed high serum IgG levels against A. fumigatus antigens. © 1990.

引用

页码：183 / 191

页数：9

共 19 条

[1]

Kurup, Resnick, Fink, A partially purified glycoprotein antigen from Aspergillus fumigatus, Int Arch Allergy Appl Immunol, 79, pp. 263-269, (1986)

[2]

Kurup, Ramasamy, Greenberger, Fink, Isolation and characterization of a relevant Aspergillus fumigatus antigen with IgG and IgE binding activity, Inl Arch Allergy Appl Immunol, 86, pp. 176-182, (1988)

[3]

Kurup, Ting, Fink, Immunochemical characterization of a major antigenic component of Aspergillus fumigatus antigens, 41, pp. 698-701, (1983)

[4]

Harvey, Longbottom, Characterization of a major antigenic component of Aspergillus fumigatus, Clin Exp Immunol, 65, pp. 206-214, (1986)

[5]

Sepulveda, Longbottom, Pepys, Enzyme linked immunosorbent assay (ELISA) for IgG and IgE antibodies to protein and polysaccharide antigens of A. fumigatus, Clin Allergy, 9, pp. 359-371, (1979)

[6]

Kauffmann, vander Laan, vander Hyden, vander Heide, Devris, Antibody determination against Aspergillus fumigatus by means of the enzyme linked immunosorbent assay 1 Liberation of polystyrene binding components during cultivation, International Archives of Allergy and Immunology, 77, pp. 343-348, (1985)

[7]

Kauffmann, Beamont, Meurs, DeHeide, Devries, Comparison of antibody measurements against Aspergillus fumigatus by means of double diffusion and enzyme linked immunosorbent assay (ELISA), Journal of Allergy and Clinical Immunology, 72, pp. 255-261, (1983)

[8]

Bradford, Rapid and sensitive method for the quantitation of protein utilizing the principles of protein dye binding, Anayt Biochem, 72, pp. 248-254, (1976)

[9]

Rosenberg, Patterson, Mintzer, Cooper, Roberts, Harris, Clinical and immunological criteria for the diagnosis of allergic bronchopulmonary aspergillosis, Ann Intern Med, 86, pp. 405-414, (1977)

[10]

Oucterloney, Antigen antibody reaction in gels types of reactions in coordinated system of diffusion, Acta Pathologica Microbiologica Scandinavica, 32, pp. 231-237, (1953)

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