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MYCOPLASMA-PNEUMONIAE DNA GYRASE GENES

被引：41
作者：
COLMAN, SD
HU, PC
BOTT, KF
机构：
[1] UNIV N CAROLINA,SCH MED,DEPT MICROBIOL & IMMUNOL,CHAPEL HILL,NC 27599
[2] UNIV N CAROLINA,SCH MED,CURRICULUM GENET,CHAPEL HILL,NC 27599
[3] UNIV N CAROLINA,SCH MED,DEPT PEDIAT,CHAPEL HILL,NC 27599
来源：
MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 07期
关键词：
D O I：
10.1111/j.1365-2958.1990.tb00687.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have identified a clone from a λEMBL3 library containing a 19kb insert of Mycoplasma pneumoniae DNA which includes the genes that encode both subunits of DNA gyrase. The gyrB gene and the 5’end of the gyrA gene have been subcloned into M13. The gryB gene is 1953bp in length and overlaps the gryA gene by a single base. The nucleotide sequence of these subclones has significant homology to previously reported gyrase genes. In terms of the size of gyrB gene and its proximity to the gyrA gene, M. pneumoniae is more similar to Bacillus subtilis than to Escherichia coli. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：1129 / 1134
页数：6
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