DETECTION OF MERCURIC IONS IN WATER BY ELISA WITH A MERCURY-SPECIFIC ANTIBODY

被引：66

作者：

WYLIE, DE

CARLSON, LD

CARLSON, R

WAGNER, FW

SCHUSTER, SM

机构：

[1] BIONEBRASKA INC,3940 CORNHUSKER HIGHWAY,SUITE 600,LINCOLN,NE 68504

[2] UNIV NEBRASKA,DEPT BIOCHEM,LINCOLN,NE 68583

[3] UNIV FLORIDA,J HILLIS MILLER HLTH CTR,DEPT BIOCHEM & MOLEC BIOL,GAINESVILLE,FL 32610

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90245-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described. The assay utilizes a monoclonal antibody that binds specifically to mercuric ions immobilized in wells of microtiter plates. Within the range of 0.5-10 ppb mercury, the absorbance in the enzyme-linked immunosorbent assay (ELISA) is linear to the log of the mercuric ion concentration. The quantitation of mercury by ELISA correlates closely with results from cold-vapor atomic absorption. Other divalent metal cations do not interfere with the assay, although there is interference in the presence of 1 mm chloride ions. The optimum pH for mercury detection is 7.0, although 2 ppb mercury can be detected over a wide pH range. The assay is as sensitive as cold-vapor atomic absorption for mercury detection and can be performed with only 100 μl of sample. © 1991.

引用

页码：381 / 387

页数：7

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