OPTIMAL NUTRIENT FEED POLICIES FOR HETEROLOGOUS PROTEIN-PRODUCTION

被引：26

作者：

HARCUM, SW ^{[1
]}

RAMIREZ, DM ^{[1
]}

BENTLEY, WE ^{[1
]}

机构：

[1] UNIV MARYLAND,DEPT CHEM ENGN,CTR AGR BIOTECHNOL,COLLEGE PK,MD 20742

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 34-5卷

关键词：

HETEROLOGOUS PROTEIN EXPRESSION; STRESS RESPONSE; PROTEASE ACTIVITY; NUTRIENT FEED POLICIES;

D O I：

10.1007/BF02920543

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant Escherichia coli, which overproduce heterologous protein, redirect endogenous metabolic activity to that mediated by the recombinant expression vector. Consequently, cells may experience perturbations in the biosynthetic reaction network, including the amino acid biosynthesis pathways. These cells are characterized by decreased growth rate, decreased cell mass yield, and increased heterologous protein degradation. This study investigates the dynamics of chloramphenicol-acetyl-transferase (CAT) synthesis and degradation in E. coli JM105 grown on minimal media, and correlates the observed phenomena with induction strength. Coordinated amino acid feeding was shown to increase the heterologous protein yield. Rational design of nutrient feeding possibilities is explored.

引用

页码：161 / 173

页数：13

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