AFFINITY OF FIBROBLAST GROWTH-FACTORS FOR BETA-CYCLODEXTRIN TETRADECASULFATE

被引：28

作者：

SHING, Y

FOLKMAN, J

WEISZ, PB

JOULLIE, MM

EWING, WR

机构：

[1] HARVARD UNIV, SCH MED, DEPT ANAT, BOSTON, MA 02115 USA

[2] HARVARD UNIV, SCH MED, DEPT CELLULAR BIOL, BOSTON, MA 02115 USA

[3] HARVARD UNIV, SCH MED, DEPT BIOL CHEM, BOSTON, MA 02115 USA

[4] UNIV PENN, DEPT CHEM & BIOENGN, PHILADELPHIA, PA 19104 USA

[5] UNIV PENN, DEPT CHEM, PHILADELPHIA, PA 19104 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90263-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

β-Cyclodextrin tetradecasulfate was found to have a very strong affinity for fibroblast growth factor (FGF) and could substitute for heparin in FGF purification. Basic FGF was purified about 200,000-fold from a rat chondrosarcoma using a method of biaffinity chromatography in which the β-cyclodextrin tetradecasulfate polymer was mixed with copper-Sepharose. This method takes advantage of the strong affinity of FGF for both β-cyclodextrin tetradecasulfate and copper. © 1990.

引用

页码：108 / 111

页数：4

共 21 条

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