CYCLIC AMP-DEPENDENT MODULATION OF CARDIAC CA CHANNELS EXPRESSED IN XENOPUS-LAEVIS OOCYTES

被引：25

作者：

LORY, P ^{[1
]}

NARGEOT, J ^{[1
]}

机构：

[1] CTR RECH BIOCHIM MACROMOLEC, CNRS, UPR 8402, INSERM, U249, ROUTE MENDE, BP 5051, F-34033 MONTPELLIER, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91839-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclic AMP-dependent modulation of cardiac L-type voltage-dependent Ca channel (VDCC) has been probed in Xenopus laevis oocytes injected with poly(A+) RNA from rat heart. A 2 to 3 fold increase of the Ba current amplitude was routinely obtained upon microinjection of cAMP (50-500 μM). Inhibition of protein kinase A (PKA) dramatically reduced the Ba current amplitude, indicating that cAMP-dependent modulation plays an important role in maintaining the basal activity of expressed Ca channels. Moreover, the effects of the DHP agonist Bay K 8644 on kinetic properties of expressed Ba current (IBa,C) were dependent on PKA activation. The results suggest that most expressed cardiac L-type VDCCs are phosphorylated and demonstrate that reconstitution in Xenopus oocytes is a suitable approach to address how phosphorylation regulates VDCC activity. © 1992.

引用

页码：1059 / 1065

页数：7

共 18 条

[1]

CATTERALL WA, 1988, J BIOL CHEM, V263, P3535

[2] EXPRESSION AND MODULATION OF VOLTAGE-GATED CALCIUM CHANNELS AFTER RNA INJECTION IN XENOPUS OOCYTES [J].