EVIDENCE FOR PANCREATIC PACEMAKER FOR INSULIN OSCILLATIONS IN LOW-FREQUENCY RANGE

被引:14
作者
CHOU, HF
BERMAN, N
IPP, E
机构
[1] UNIV CALIF LOS ANGELES, LOS ANGELES CTY HARBOR MED CTR, DEPT MED, DIV ENDOCRINOL, TORRANCE, CA 90502 USA
[2] UNIV CALIF LOS ANGELES, LOS ANGELES CTY HARBOR MED CTR, DEPT PEDIAT, TORRANCE, CA 90502 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 266卷 / 06期
关键词
ISLETS OF LANGERHANS; PULSATILE INSULIN SECRETION; ULTRADIAN OSCILLATIONS;
D O I
10.1152/ajpregu.1994.266.6.R1786
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Circulating insulin concentrations oscillate in regular fashion, with periods that fall into a high-frequency (period of 5-17 min) or low-frequency (period of 50-150 min) range. Only the high frequency oscillations have so far been reported in vitro, suggesting that these derive from a primary pancreatic source. This study tested whether the low-frequency insulin oscillations could also be identified in vitro. Rat islets of Langerhans were perifused for 20 h using RPMI medium with 5.5 mM glucose. Perifusate fractions were collected at 9.9-min intervals. Mean insulin concentrations at the outset were 21.4 +/- 2.9 mu U/ml, increased to 32.5 +/- 4.6 (P < 0.05) between 13 and 17 h after the start of perifusion, and then either leveled off or decreased to baseline. Superimposed on this general trend, we found sustained insulin oscillations with a period of 50-100 min. The mean amplitude was 14.2 +/- 4.2 mu U/ml, and the amplitude/mean ratio was 64.6 +/- 12%. Spectral analysis revealed significant peaks at periods that were close to either 50 or 100 min and a smaller peak at 24-37 min. These data, using in vitro methodology and constant glucose concentrations, indicate the presence of sustained, spontaneous, low-frequency, ultradian insulin oscillations in the pancreatic islets. This provides evidence for a pancreatic component that may participate in the previously described in vivo ultradian insulin oscillations. This finding may also provide a mechanism for the apparent escape from glucose entrainment of serum insulin oscillations in non-insulin-dependent diabetes mellitus.
引用
收藏
页码:R1786 / R1791
页数:6
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