SELECTIVE KILLING OF MURINE LEUKEMIC-CELLS BY ADENOSINE-TRIPHOSPHATE (ATP) - A STUDY OF THE VALUE OF AUTOLOGOUS BONE-MARROW TRANSPLANTATION

被引：6

作者：

HATTA, Y

AIZAWA, S

HORIKOSHI, A

BABA, M

HORIE, T

机构：

[1] The First Department of Internal Medicine, Nihon University School of Medicine, Tokyo

[2] The First Department of Internal Medicine, Tokyo Medical College, Tokyo

来源：

INTERNAL MEDICINE | 1993年 / 32卷 / 10期

关键词：

WEHI3B; L1210; PURGING; HEMATOPOIETIC STEM CELL; CELL MEMBRANE; PERMEABILITY;

D O I：

10.2169/internalmedicine.32.768

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

To assess the value of adenosine triphosphate (ATP) for ex vivo purging of leukemic cells in autologous bone marrow transplantation, its biological effects on the murine leukemic cell lines (WEHI3B and L1210) and normal murine bone marrow hemopoietic stem cells (CFU-C and CFU-S) were studied. After treatment with 4 mM of ATP for 6 h, the number of viable WEHI3B cells decreased to less than 0.1% of that of the control. Furthermore, H-3-thymidine incorporations were also completely inhibited in both WEHI3B cells and L1210 cells. These phenomena were related to the concentration and exposure period of ATP. Treatment of bone marrow mononuclear cells with ATP under the same condition reduced the number of CFU-C, day 9 CFU-S and day 12 CFU-S to only 58.5 +/- 8.7%, 92.6 +/- 8.2% and 83.5 +/- 28.5%, respectively, with no change in the number of marrow nucleated cells. Although the effect of ATP is not entirely specific to leukemic cells, these findings provide evidence that ATP is useful for purging residual tumor cells in autologous bone marrow transplantation.

引用

页码：768 / 772

页数：5

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