TOWARDS THE INTEGRATION OF FOREIGN DNA INTO THE CHROMOSOME OF MYCOBACTERIUM-LEPRAE

被引：8

作者：

EIGLMEIER, K ^{[1
]}

HONORE, N ^{[1
]}

COLE, ST ^{[1
]}

机构：

[1] INST PASTEUR,GENET MOLEC BACTERIENNE LAB,F-75724 PARIS 15,FRANCE

来源：

RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 06期

关键词：

MYCOBACTERIUM-LEPRAE; GENOME MAPPING; INTEGRATIVE VECTOR; TRANSFER RNA; PLASMID; LEPROSY; MYCOBACTERIA; PSAM2; SITE-SPECIFIC INTEGRATION; STREPTOMYCES-AMBOFACIENS; PLASMID PSAM2; CONSTRUCTION; EXPRESSION; VECTOR;

D O I：

10.1016/0923-2508(91)90074-K

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Integrative plasmid vectors based on the pSAM2 system of Streptomyces ambofaciens offer great potential for the genetic analysis of Mycobacterium leprae. To assess this, the chromosomal attachment site of M. leprae, att-pSAM2, has been cloned, mapped and characterized. Nucleotide sequence analysis shows att-pSAM2 to correspond to a putative tRNA(pro) gene identical in sequence to those of S. ambofaciens and M. tuberculosis. In addition, it is shown that the genes encoding aspartate semialdehyde dehydrogenase, asd, and an anonymous protein antigen recognized by sera from leprosy patients, are linked to the M. leprae att-pSAM2 locus.

引用

页码：617 / 622

页数：6

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