MONITORING OF YERSINIA-ENTEROCOLITICA IN MURINE AND BOVINE FECES ON THE BASIS OF THE CHROMOSOMALLY INTEGRATED LUXAB MARKER GENE

被引：13

作者：

KANIGA, K

SORY, MP

DELOR, I

SAEGERMAN, C

LIMET, JN

CORNELIS, GR

机构：

[1] CATHOLIC UNIV LOUVAIN, INT INST CELLULAR & MOLEC PATHOL, MICROBIAL PATHOGENESIS UNIT, B-1200 BRUSSELS, BELGIUM

[2] CATHOLIC UNIV LOUVAIN, FAC MED, B-1200 BRUSSELS, BELGIUM

[3] FAC UNIV NOTRE DAME PAIX, IMMUNOL & MICROBIOL LAB, B-5000 NAMUR, BELGIUM

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 03期

关键词：

D O I：

10.1128/AEM.58.3.1024-1026.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We previously integrated the luxAB gene into the Yersinia enterocolitica chromosome. In this article, we assessed, by luminometry, the survival of the engineered strain KNG1024 in the digestive tracts of mice and cows. In situ detection and a count of the released strain were performed on feces from orally inoculated BALB/c mice for 24 days. This method is a rapid and reliable system for long-term monitoring of genetically engineered bacteria. In cow feces, the count of Y. enterocolitia ranged from 210 to 6,000 CFU/g of feces. This very low count was not detectable by direct luminometry.

引用

页码：1024 / 1026

页数：3

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