CLOSE CORRELATION BETWEEN HEAT-SHOCK RESPONSE AND CYTOTOXICITY IN NEUROSPORA-CRASSA TREATED WITH ALIPHATIC-ALCOHOLS AND PHENOLS

被引:21
作者
MEYER, U [1 ]
SCHWEIM, P [1 ]
FRACELLA, F [1 ]
RENSING, L [1 ]
机构
[1] UNIV BREMEN, INST CELL BIOL BIOCHEM & BIOTECHNOL, D-28334 BREMEN, GERMANY
关键词
D O I
10.1128/AEM.61.3.979-984.1995
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In Neurospora crassa the aliphatic alcohols methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, isobutanol, ethylene glycol, glycerol, and allyl alcohol and the phenolic compounds phenol, hydroquinone, resorcinol, pyrogallol, phloroglucinol, sodium salicylate, and acetylsalicylic acid were analyzed with respect to their capacities to induce heat shock proteins (HSP) and to inhibit protein synthesis, Both the alcohols and phenols showed the greatest levels of HSP induction at concentrations which inhibited the overall protein synthesis by about 50%. The abilities of the different alcohols to induce the heat shock response are proportional to their lipophilicities: the lipophilic alcohol isobutanol is maximally inductive at about 0.6 hi, whereas the least lipophilic alcohol, methanol, causes maximal induction at 5.7 M. The phenols, in general, show a higher capability to induce the heat shock response, The concentrations for maximal induction range between 25 mM (sodium salicylate) and 100 mM (resorcinol), Glycerol (4.1 M) shifted the concentration necessary for maximal HSP induction by hydroquinone from 50 to 200 mM. The results reveal that the induction of HSP occurs under conditions which considerably constrain cell metabolism, The heat shock response, therefore, does not represent a sensitive marker for toxicity tests but provides a good estimate for the extent of cell damage.
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页码:979 / 984
页数:6
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