CONTRIBUTION TO PROBLEM OF CLONATION OF LYMPHATIC CELLS IN VIVO

In transfers of lymph node or thymus cells from normal mice to irradiated recipients the application of phytohemagglutinin to the donors had no significant effect on proliferation of lymphatic elements. Phytohemagglutinin only partially stimulated hemopoiesis after transfer of spleen cells. On transfers of allogeneic lymphatic cells there is marked proliferation in the lymphatic organs having the character of the graft-versus-host reaction. For clonation of lymphatic cells a very suitable procedure was used in which the mouse spleen cells were transferred to syngeneic supralethally irradiated recipients which at the same time received as antigen sheep, or also rabbit erythrocytes. The clones of antibody producing cells were detected by the hemolytic activity of spleen fragments placed between two agarose plates with sheep and rabbit erythrocytes. © 1968 Institute of Microbiology, Academy of Sciences of the Czech Republic.