ALPHA-2-MACROGLOBULIN IS AN ASTROGLIA-DERIVED NEURITE-PROMOTING FACTOR FOR CULTURED NEURONS FROM RAT CENTRAL-NERVOUS-SYSTEM

被引：46

作者：

MORI, T ^{[1
]}

IIJIMA, N ^{[1
]}

KITABATAKE, K ^{[1
]}

KOHSAKA, S ^{[1
]}

机构：

[1] NATL INST NEUROSCI,DEPT NEUROCHEM,TOKYO,JAPAN

来源：

BRAIN RESEARCH | 1990年 / 527卷 / 01期

关键词：

Astroglia; Neurite-promoting factor; Neuron; α[!sub]2[!/sub]-Macroglobulin;

D O I：

10.1016/0006-8993(90)91059-P

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The neurite promoting factors in the astroglial conditioned medium (As-CM) were characterized by using primary cultures of embryonic rat neocortical neurons. The factors in the As-CM bind to lectins such as wheat germ agglutinin (WGA), suggesting that they contain sugar moieties. When the WGA-bound fractions were applied on a Superose 6 column, the activity was recovered mainly in two fractions, peak I and peak II. The peak II fraction was further purified by Mono Q anion exchange chromatography. A single protein band of 180 kDa was detected in the final Mono Q fraction by sodium dodesylsulfate polyacrylamide gel electrophoresis. The molecular weight coincided with that of α2-macroglobulin (α2M). Western blotting showed that the single protein band was reacted with anti-α2M antibody but not with anti-fibronectin and anti-laminin antisera. The neurite-promoting activity of the Mono Q fraction was inhibited by anti-α2M antibody. Furthermore, commercially available α2M also promotes neurite outgrowth in our assay system. These results strongly suggested that α2M is one of the neurite-promoting factors in the As-CM. © 1990.

引用

页码：55 / 61

页数：7

共 38 条

[1] REGULATION OF SYNTHESIS AND SECRETION OF MAJOR RAT ACUTE-PHASE PROTEINS BY RECOMBINANT HUMAN INTERLEUKIN-6 (BSF-2/IL-6) IN HEPATOCYTE PRIMARY CULTURES [J].