A GENERAL STRATEGY FOR THE ISOLATION OF MESOPHYLL PROTOPLASTS FROM DECIDUOUS FRUIT AND NUT TREE SPECIES

被引：25

作者：

REVILLA, MA

OCHATT, SJ

DOUGHTY, S

POWER, JB

机构：

[1] UNIV NOTTINGHAM, DEPT BOT, PLANT GENET MANIPULAT GRP, NOTTINGHAM NG7 2RD, ENGLAND

[2] UNIV OVIEDO, FAC BIOL, DEPT FISIOL VEGETAL, OVIEDO, SPAIN

来源：

PLANT SCIENCE | 1987年 / 50卷 / 02期

关键词：

D O I：

10.1016/0168-9452(87)90149-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme mixture of general applicability, containing 1.0% Onozuka R-10, 1.0% Hemicellulase, and 0.1% Pectolyase Y-23 provided the basis for the isolation of large numbers of viable mesophyll protoplasts from woody species within the families Betulaceae, Juglandaceae, Rosaceae and Rutaceae. This is the first report of protoplast isolation from leaves of hazelnut, walnut, sweet cherry, sour cherry and the common pear.

引用

页码：133 / 137

页数：5

共 27 条

[1]

Ahuja M R, 1983, SILVAE GENET, V32, P5

[2]

AHUJA MR, 1984, SILVAE GENET, V33, P1

[3] A GENERAL-METHOD FOR THE HIGH-YIELD ISOLATION OF MESOPHYLL PROTOPLASTS FROM DECIDUOUS TREE SPECIES [J].