ELECTRON-MICROSCOPY AND IMAGE-ANALYSIS OF 2-DIMENSIONAL CRYSTALS AND SINGLE MOLECULES OF ALCOHOL OXIDASE FROM HANSENULA-POLYMORPHA

被引：45

作者：

VONCK, J ^{[1
]}

VANBRUGGEN, EFJ ^{[1
]}

机构：

[1] STATE UNIV GRONINGEN,BIOCHEM LAB,NIJENBORGH 16,9747 AG GRONINGEN,NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1038卷 / 01期

关键词：

(H. polymorpha); Alcohol oxidase; Electron microscopy; Image analysis;

D O I：

10.1016/0167-4838(90)90012-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The octameric protein alcohol oxidase from the yeast Hansenula polymorpha was studied by electron microscopy and image analysis. Two-dimensional crystals were formed by applying the protein, in a phosphate buffer containing poly(ethylene glycol) and EDTA, to a carbon-coated formvar film which had been glow-discharged in pentylamine at least several hours earlier. The crystals show p4 symmetry and have a unit cell of 12.5 × 12.5 nm2, containing one molecule. Image analysis of the crystals and of single molecules yielded two different views. From these it can be deduced that the subunits have an elongated shape and form two layers of four, stacked face to face. A tentative model of the structure is presented. © 1990.

引用

页码：74 / 79

页数：6

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