RAPID ANALYSIS OF CAMPYLOBACTER ISOLATES - DNA FINGERPRINTING WITHOUT THE NEED TO PURIFY DNA

被引：128

作者：

MAZURIER, S ^{[1
]}

VANDEGIESSEN, A ^{[1
]}

HEUVELMAN, K ^{[1
]}

WERNARS, K ^{[1
]}

机构：

[1] NATL INST PUBL HLTH & ENVIRONM PROTECT,WATER & FOOD MICROBIOL LAB,POB 1,3720 BA BILTHOVEN,NETHERLANDS

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1992年 / 14卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1992.tb00700.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.

引用

页码：260 / 262

页数：3

共 4 条

[1] SEROTYPING OF CAMPYLOBACTER-JEJUNI BY SLIDE AGGLUTINATION BASED ON HEAT-LABILE ANTIGENIC FACTORS
LIOR, H
WOODWARD, DL
EDGAR, JA
LAROCHE, LJ
GILL, P
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (05) : 761 - 768
[2] MAZURIER S, 1992, IN PRESS APPLIED ENV
[3] EVALUATION OF 10 METHODS TO DISTINGUISH EPIDEMIC-ASSOCIATED CAMPYLOBACTER STRAINS
PATTON, CM
WACHSMUTH, IK
EVINS, GM
KIEHLBAUCH, JA
PLIKAYTIS, BD
TROUP, N
TOMPKINS, L
LIOR, H
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (04) : 680 - 688
[4] PASSIVE HEMAGGLUTINATION TECHNIQUE FOR SEROTYPING CAMPYLOBACTER-FETUS SUBSP-JEJUNI ON THE BASIS OF SOLUBLE HEAT-STABLE ANTIGENS
PENNER, JL
HENNESSY, JN
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1980, 12 (06) : 732 - 737

← 1 →