PROLINE FOR ALANINE SUBSTITUTIONS IN THE C-PEPTIDE HELIX OF RIBONUCLEASE-A

被引：49

作者：

STREHLOW, KG ^{[1
]}

ROBERTSON, AD ^{[1
]}

BALDWIN, RL ^{[1
]}

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT BIOCHEM,STANFORD,CA 94305

来源：

BIOCHEMISTRY | 1991年 / 30卷 / 23期

关键词：

D O I：

10.1021/bi00237a026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect on overall alpha-helix content of substituting proline for alanine has been determined at 5 positions ( 1, 2, 4, 5, and 13) of a 13-residue peptide related in sequence to residues 1-1 3 of ribonuclease A. The helix content falls off rapidly as proline is moved inward, and the proline residue effectively truncates the helix. No helix-stabilizing effect of proline is found at positions 2 or 4 within the first turn of the helix. Proline substitution at either end position (1, 13) has little effect on overall helix content, in agreement with an earlier study of glycine for alanine substitutions. The two end residues of the helix appear to be strongly frayed.

引用

页码：5810 / 5814

页数：5

共 22 条

[1] HELIX COIL STABILITY-CONSTANTS FOR THE NATURALLY-OCCURRING AMINO-ACIDS IN WATER .23. PROLINE PARAMETERS FROM RANDOM POLY(HYDROXYBUTYLGLUTAMINE-CO-L-PROLINE) [J].

ALTMANN, KH ;

WOJCIK, J ;

VASQUEZ, M ;

SCHERAGA, HA .

BIOPOLYMERS, 1990, 30 (1-2) :107-120

[2] DERIVATIVE SPECTROSCOPY APPLIED TO TYROSYL CHROMOPHORES - STUDIES ON RIBONUCLEASE, LIMA BEAN INHIBITORS, INSULIN, AND PANCREATIC TRYPSIN-INHIBITOR [J].

BRANDTS, JF ;

KAPLAN, LJ .

BIOCHEMISTRY, 1973, 12 (10) :2011-2024

[3]

CHAKRABARTTY A, 1991, IN PRESS NATURE

[4] DETERMINATION OF HELIX AND BETA-FORM OF PROTEINS IN AQUEOUS-SOLUTION BY CIRCULAR-DICHROISM [J].

CHEN, YH ;

YANG, JT ;

CHAU, KH .

BIOCHEMISTRY, 1974, 13 (16) :3350-3359

[5] THE GLU-2-=ARG-10+ SIDE-CHAIN INTERACTION IN THE C-PEPTIDE HELIX OF RIBONUCLEASE-A [J].

FAIRMAN, R ;

SHOEMAKER, KR ;

YORK, EJ ;

STEWART, JM ;

BALDWIN, RL .

BIOPHYSICAL CHEMISTRY, 1990, 37 (1-3) :107-119

[6] FURTHER-STUDIES OF THE HELIX DIPOLE MODEL - EFFECTS OF A FREE ALPHA-NH3+ OR ALPHA-COO- GROUP ON HELIX STABILITY [J].

FAIRMAN, R ;

SHOEMAKER, KR ;

YORK, EJ ;

STEWART, JM ;

BALDWIN, RL .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1989, 5 (01) :1-7

[7] A HELIX STOP SIGNAL IN THE ISOLATED S-PEPTIDE OF RIBONUCLEASE-A [J].

KIM, PS ;

BALDWIN, RL .

NATURE, 1984, 307 (5949) :329-334

[8] THEORY OF HELIX-COIL TRANSITION IN POLYPEPTIDES [J].

LIFSON, S .

JOURNAL OF CHEMICAL PHYSICS, 1961, 34 (06) :1963-&

[9] HELIX STABILIZATION BY GLU- ... LYS+ SALT BRIDGES IN SHORT PEPTIDES OF DENOVO DESIGN [J].

MARQUSEE, S ;

BALDWIN, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) :8898-8902

[10] UNUSUALLY STABLE HELIX FORMATION IN SHORT ALANINE-BASED PEPTIDES [J].

MARQUSEE, S ;

ROBBINS, VH ;

BALDWIN, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5286-5290

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