IMPROVEMENT OF CHEMICAL-ANALYSIS OF ANTIBIOTICS .18. IDENTIFICATION OF RESIDUAL TETRACYCLINES IN BOVINE-TISSUES BY TLC FABMS WITH A SAMPLE CONDENSATION TECHNIQUE

被引:22
作者
OKA, H
IKAI, Y
HAYAKAWA, J
MASUDA, K
HARADA, K
SUZUKI, M
MARTZ, V
MACNEIL, JD
机构
[1] MEIJO UNIV, FAC PHARM, NAGOYA 468, JAPAN
[2] AGR CANADA, HLTH ANIM LAB, SASKATOON S7N 2R3, SASKATCHEWAN, CANADA
关键词
D O I
10.1021/jf00027a013
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
To reliably identify residual tetracycline antibiotics (TCs) oxytetracycline, tetracycline, chlortetracycline, and doxycycline in bovine tissues, a confirmation method using thin-layer chromatography/fast atom bombardment mass spectrometry (TLC/FABMS) has been established. This method consists of the following four steps: (1) extraction of TCs from bovine tissues with McIlvaine buffer (pH 4.0) containing 0.1 M ethylenediaminetetraacetate (Na2EDTA); (2) elution of the TCs from prepacked C18 cartridges, pretreated with Na2EDTA, with ethyl acetate followed by methanol-ethyl acetate (5:95 v/v) as eluents; (3) separation of the TCs on a reversed-phase C-8 TLC plate with methanol-acetonitrile-0.5 M oxalic acid solution (pH 2.0) (1:1:4 v/v) as a solvent system; and (4) TLC/FABMS with a condensation technique employing thioglycerol as a matrix. The method can reliably identify TCs fortified at concentrations of 0.1 ppm in bovine tissues, including muscle, liver, and kidney, and has been successfully applied to the identification of residual TCs in bovine incurred samples.
引用
收藏
页码:410 / 415
页数:6
相关论文
共 11 条
  • [1] SEPARATION AND IDENTIFICATION OF FOOD DYES BY THIN-LAYER CHROMATOGRAPHY LIQUID SECONDARY ION MASS-SPECTROMETRY
    HARADA, K
    MASUDA, K
    SUZUKI, M
    OKA, H
    [J]. BIOLOGICAL MASS SPECTROMETRY, 1991, 20 (09) : 522 - 528
  • [2] IMPROVEMENT OF CHEMICAL-ANALYSIS OF ANTIBIOTICS .13. SYSTEMATIC SIMULTANEOUS ANALYSIS OF RESIDUAL TETRACYCLINES IN ANIMAL-TISSUES USING THIN-LAYER AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    IKAI, Y
    OKA, H
    KAWAMURA, N
    YAMADA, M
    HARADA, K
    SUZUKI, M
    [J]. JOURNAL OF CHROMATOGRAPHY, 1987, 411 : 313 - 323
  • [3] KENION GB, 1990, ACS SYM SER, V420, P140
  • [4] CONFIRMATION OF OXYTETRACYCLINE, TETRACYCLINE AND CHLORTETRACYCLINE RESIDUES IN MILK BY PARTICLE BEAM LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY
    KIJAK, PJ
    LEADBETTER, MG
    THOMAS, MH
    THOMPSON, EA
    [J]. BIOLOGICAL MASS SPECTROMETRY, 1991, 20 (12) : 789 - 795
  • [5] DETERMINATION OF RESIDUES OF TETRACYCLINE ANTIBIOTICS IN ANIMAL-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    MULDERS, EJ
    VANDELAGEMAAT, D
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1989, 7 (12) : 1829 - 1835
  • [6] OKA H, 1991, J ASSOC OFF ANA CHEM, V74, P894
  • [7] DEVELOPMENT OF A CONDENSATION TECHNIQUE FOR THIN-LAYER CHROMATOGRAPHY FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY OF NON-VISIBLE COMPOUNDS
    OKA, H
    IKAI, Y
    KONDO, F
    KAWAMURA, N
    HAYAKAWA, J
    MASUDA, K
    HARADA, K
    SUZUKI, M
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (02) : 89 - 94
  • [8] IMPROVEMENT OF CHEMICAL-ANALYSIS OF ANTIBIOTICS .5. A SIMPLE METHOD FOR THE ANALYSIS OF TETRACYCLINES USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    OKA, H
    UNO, K
    HARADA, KI
    YASAKA, K
    SUZUKI, M
    [J]. JOURNAL OF CHROMATOGRAPHY, 1984, 298 (03): : 435 - 443
  • [9] IMPROVEMENT OF CHEMICAL-ANALYSIS OF ANTIBIOTICS .3. SIMPLE METHOD FOR THE ANALYSIS OF TETRACYCLINES ON REVERSED-PHASE THIN-LAYER PLATES
    OKA, H
    UNO, K
    HARADA, K
    SUZUKI, M
    [J]. JOURNAL OF CHROMATOGRAPHY, 1984, 284 (01): : 227 - 234
  • [10] IMPROVEMENT OF THE CHEMICAL-ANALYSIS OF ANTIBIOTICS .1. SIMPLE METHOD FOR THE ANALYSIS OF TETRACYCLINES ON SILICA-GEL HIGH-PERFORMANCE THIN-LAYER PLATES
    OKA, H
    UNO, K
    HARADA, KI
    KANEYAMA, Y
    SUZUKI, M
    [J]. JOURNAL OF CHROMATOGRAPHY, 1983, 260 (02): : 457 - 462