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MAGNETIC CIRCULAR-DICHROISM STUDY OF THE SELENIUM-SUBSTITUTED FORM (FE FE3SE4) OF BOVINE HEART ACONITASE

被引：8
作者：
BRETON, JL
FARRAR, JA
KENNEDY, MC
BEINERT, H
THOMSON, AJ
机构：
[1] MED COLL WISCONSIN,DEPT BIOCHEM,MILWAUKEE,WI 53226
[2] MED COLL WISCONSIN,BIOPHYS RES INST,MILWAUKEE,WI 53226
[3] UNIV WISCONSIN,INST ENZYME RES,MADISON,WI 53705
来源：
BIOCHEMICAL JOURNAL | 1995年 / 311卷
关键词：
D O I：
10.1042/bj3110197
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The selenium-substituted inactive form of mitochondrial aconitase contains one [3Fe-4Se](1+/0) cluster [Surerus, Kennedy, Beinert and Munck (1989) Proc. Natl. Acad. Sci, U.S.A. 87, 9846-9850]. This cluster was studied in both oxidized and reduced states by magnetic CD (MCD) and EPR spectroscopy. In the MCD spectra, intensity and transition wavelength shifts are observed when compared with the spectra of the native [3Fe-4S](1+0) cluster. These changes are used to differentiate between the charge transfer transitions originating from inorganic and cysteinyl sulphur. Using also the data from the EPR spectra, the spin ground state is assigned as S = 1/2 for the oxidized [3Fe-4Se](1+) cluster and S = 2 for the reduced [3Fe-4Se](0) cluster.
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页码：197 / 202
页数：6
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