THE RATE OF TETANIC RELAXATION IS CORRELATED WITH THE DENSITY OF CALCIUM ATPASE IN THE TERMINAL CISTERNAE OF THYROTOXIC SKELETAL-MUSCLE

The density of calcium ATPase was measured in the terminal cisternae of extensor digitorum longus (EDL) and soleus muscles from normal and thyrotoxic rats. The experiments tested the hypothesis that the rate of relaxation of these muscles following contraction, at temperatures above 22°C, is correlated with the density of calcium ATPase in the sarcoplasmic reticular membrane. In soleus fibres there was a progressive increase in calcium ATPase density, measured with immuno-electronmicroscopic techniques, of more than two-fold after 3-weeks of daily injections with triiodothyronine (T3). There was a parallel decrease in the relaxation time (from 80% to 20% of peak tension) of the tetanus: the parameters were closely correlated (r=0.998) during the 3-week period. The rate of relaxation of the twitch also doubled and was correlated with the increase in gold particle density at the end of the 3-week injection period. However, twitch relaxation slowed during the 1st week of T3 injection and was not correlated with gold particle density at that time. The changes in calcium ATPase density and relaxation times in EDL fibres were small and largely insignificant. In contrast to relaxation, an increase in the rate of rise of tension is soleus was complete after only 2 weeks of T3 injection. The results show that the relaxation of tetanic tension is closely correlated with the calcium uptake capacity of the sarcoplasmic reticulum and that thyroid hormone acts more rapidly on factors regulating the rate of rise of tension than on those regulating tension relaxation and the density of calcium ATPase in the terminal cisternae. © 1990 Springer-Verlag.