PROTEIN-A FUSION VECTORS FOR USE IN COMBINATION WITH PEX VECTORS IN THE PRODUCTION AND AFFINITY PURIFICATION OF SPECIFIC ANTIBODIES

被引：10

作者：

ZUECO, J ^{[1
]}

BOYD, A ^{[1
]}

机构：

[1] UNIV EDINBURGH, SCH MED, DEPT BIOCHEM, GEORGE SQ, EDINBURGH EH8 9XD, SCOTLAND

来源：

GENE | 1992年 / 121卷 / 01期

基金：

英国医学研究理事会;

关键词：

RECOMBINANT DNA; BETA-GALACTOSIDASE; ESCHERICHIA-COLI; KANAMYCIN RESISTANCE; RABBIT ANTIBODIES;

D O I：

10.1016/0378-1119(92)90180-W

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

New vectors expressing protein A fusions were constructed, based upon the kanamycin-resistance-encoding plasmid, pK19. They were designed for use in combination with the pEX series of beta-galactosidase protein-fusion vectors in the production and affinity purification of specific antibodies.

引用

页码：181 / 182

页数：2

共 8 条

[1]

KOERNER TJ, 1991, METHOD ENZYMOL, V194, P477

[2] IMPROVEMENT OF THE CLONING LINKER OF THE BACTERIAL EXPRESSION VECTOR PEX [J].

KUSTERS, JG ;

JAGER, EJ ;

VANDERZEIJST, BAM .

NUCLEIC ACIDS RESEARCH, 1989, 17 (19) :8007-8007

[3] PRODUCTION OF SPECIFIC ANTIBODIES AGAINST PROTEIN-A FUSION PROTEINS [J].

LOWENADLER, B ;

NILSSON, B ;

ABRAHMSEN, L ;

MOKS, T ;

LJUNGQVIST, L ;

HOLMGREN, E ;

PALEUS, S ;

JOSEPHSON, S ;

PHILIPSON, L ;

UHLEN, M .

EMBO JOURNAL, 1986, 5 (09) :2393-2398

[4]

MARSTON FAO, 1990, METHOD ENZYMOL, V182, P264

[5] NEW AND VERSATILE CLONING VECTORS WITH KANAMYCIN-RESISTANCE MARKER [J].

PRIDMORE, RD .

GENE, 1987, 56 (2-3) :309-312

[6]

PRINGLE JR, 1991, METHOD ENZYMOL, V194, P565

[7] SINGLE-STEP PURIFICATION OF POLYPEPTIDES EXPRESSED IN ESCHERICHIA-COLI AS FUSIONS WITH GLUTATHIONE S-TRANSFERASE [J].

SMITH, DB ;

JOHNSON, KS .

GENE, 1988, 67 (01) :31-40

[8]

STANLEY K, 1984, EMBO J, V84, P1429

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