LUMINESCENCE STUDY OF UO-2(2+) BINDING TO IMMOBILIZED DATURA-INNOXIA BIOMATERIAL

The spectrally and temporally resolved UO2(2+) fluorescence signals have been measured for solid samples of UO2(2+)-Datura, UO2(2+)-immobilized Datura), and UO2(2+)-(silicate polymer) at liquid nitrogen temperature. The binding capacity of UO2(2+) to Datura innoxia cell material has been enhanced significantly at pH 5 and 6 when immobilized in a polysilicate matrix. New binding sites having a greater binding strength with a lower availability have been observed for the binding of UO2(2+) after immobilization of the cultured biomaterial. However, chemical alteration of the cell material resulting from this immobilization process has not been observed. The chemical environment of the binding sites responsible for the binding of UO2(2+) in immobilized and free D. innoxia cell material has been demonstrated to be different. This difference results from the immobilization of the cell material within a polysilicate matrix.