EVALUATION OF THE SENSITIVITY AND SPECIFICITY OF POLYMERASE CHAIN-REACTION TEST KIT, AMPLICOR CHLAMYDIA-TRACHOMATIS

被引：16

作者：

MIYASHITA, N ^{[1
]}

IIJIMA, Y ^{[1
]}

MATSUMOTO, A ^{[1
]}

机构：

[1] KAWASKAI MED SCH,DEPT MICROBIOL,KURASHIKI,OKAYAMA 70101,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 38卷 / 01期

关键词：

POLYMERASE CHAIN REACTION; CHLAMYDIA TRACHOMATIS; ELEMENTARY BODIES; SENSITIVITY;

D O I：

10.1111/j.1348-0421.1994.tb01747.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The sensitivity and specificity of the polymerase chain reaction (PCR) test kit, AMPLICOR Chlamydia trachomatis, were examined by the use of purified elementary bodies (EBs), cells having inclusions containing reticulate bodies alone and 20 clinical isolates. The numbers of EB and inclusion of C. trachomatis at the detection limit were determined to be approximately 2 to 4 EBs and one inclusion per assay, respectively. No reaction occurred for C. psittaci and C. pneumoniae. All clinical isolates were positively reacted in the PCR assay.

引用

页码：81 / 85

页数：5

共 12 条

[1] CHARACTERIZATION OF THE NEW CHLAMYDIA AGENT, TWAR, AS A UNIQUE ORGANISM BY RESTRICTION ENDONUCLEASE ANALYSIS AND DNA-DNA HYBRIDIZATION
CAMPBELL, LA
KUO, CC
GRAYSTON, JT
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (10) : 1911 - 1916
[2] An unidentified virus producing acute meningitis and pneumonitis in experimental animals
Francis, T
Magill, TP
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1938, 68 (02) : 147 - U3
[3] A NEW CHLAMYDIA-PSITTACI STRAIN, TWAR, ISOLATED IN ACUTE RESPIRATORY-TRACT INFECTIONS
GRAYSTON, JT
KUO, CC
WANG, SP
ALTMAN, J
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1986, 315 (03) : 161 - 168
[4] DETECTION OF CHLAMYDIA-TRACHOMATIS IN ENDOCERVICAL SPECIMENS BY POLYMERASE CHAIN-REACTION
LOEFFELHOLZ, MJ
LEWINSKI, CA
SILVER, SR
PUROHIT, AP
HERMAN, SA
BUONAGURIO, DA
DRAGON, EA
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) : 2847 - 2851
[5] LOVETT M, 1980, CURRENT CHEMOTHERAPY, V2, P1250
[6] MATUMOTO MINORU, 1957, JAPANESE JOUR EXPTL MED, V27, P191
[7] ESTABLISHMENT OF A PARTICLE-COUNTING METHOD FOR PURIFIED ELEMENTARY BODIES OF CHLAMYDIAE AND EVALUATION OF SENSITIVITIES OF THE IDEIA CHLAMYDIA KIT AND DNA PROBE BY USING THE PURIFIED ELEMENTARY BODIES
MIYASHITA, N
MATSUMOTO, A
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) : 2911 - 2916
[8] A COMMON PLASMID OF CHLAMYDIA-TRACHOMATIS
PALMER, L
FALKOW, S
[J]. PLASMID, 1986, 16 (01) : 52 - 62
[9] THE 7.5-KB PLASMID PRESENT IN CHLAMYDIA-TRACHOMATIS IS NOT ESSENTIAL FOR THE GROWTH OF THIS MICROORGANISM
PETERSON, EM
MARKOFF, BA
SCHACHTER, J
DELAMAZA, LM
[J]. PLASMID, 1990, 23 (02) : 144 - 148
[10] POPOV VL, 1991, FEMS MICROBIOL LETT, V84, P129

← 1 2 →