EFFECT OF THE NUMBER OF INSEMINATED SPERMATOZOA ON SUBSEQUENT HUMAN AND MOUSE EMBRYONIC-DEVELOPMENT INVITRO

It has been shown, in both human and mouse in-vitro fertilization (IVF), that an excess number of spermatozoa in the insemination medium leads to reduced fertilization rates. In this study, we evaluated human embryonic development after dividing the oocytes of each of 62 IVF attempts into two groups on the basis of insemination with two widely used concentrations (50 000 and 100 000 spermatozoa/ml).The embryonic growth was retarded in the group inseminated with 100 000 spermatozoa/ml: significantly fewer fast developing embryos (4-cell and 5- to 8-cell stages) were found (53.4% in the 100 000/ml group and 65.5% in the 50 000 group; P < 0.05). In two experimental series, mouse embryonic development was evaluated in the presence of 0, 50 000, 100 000 and 500 000 spermatozoa per ml. In the first series, the spermatozoa were present during 5 - 20 h after insemination, while in the second series, the spermatozoa were present during the whole culture period of 120 h. The development of mouse embryos was impaired when 500 000/ml spermatozoa were present during the whole culture period. In contrast with human IVF results, the presence of up to 500 000 spermatozoa during the first 20 h after insemination did not have any significant detrimental effect on blastocyst formation in the mouse.