OLIGONUCLEOTIDE PROBES DETECT SPLICING VARIANTS INSITU IN DROSOPHILA EMBRYOS

被引：18

作者：

ARTERO, RD ^{[1
]}

AKAM, M ^{[1
]}

PEREZALONSO, M ^{[1
]}

机构：

[1] WELLCOME CRC,INST CANC & DEV BIOL,CAMBRIDGE CB2 1QR,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 21期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/20.21.5687

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and IIa are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.

引用

页码：5687 / 5690

页数：4

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