MESSENGER-RNA 3'-END FORMATION OF A DNA FRAGMENT FROM THE HUMAN C-MYC 3'-END REGION IN SACCHAROMYCES-CEREVISIAE

被引:5
作者
IRNIGER, S [1 ]
EGLI, CM [1 ]
BRAUS, GH [1 ]
机构
[1] SWISS FED INST TECHNOL,INST MICROBIOL,SCHMELZBERGSTR 7,CH-8092 ZURICH,SWITZERLAND
关键词
POLYADENYLATION; RNA 3'-END FORMATION; TRANSCRIPTION TERMINATION; YEAST;
D O I
10.1007/BF00351496
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We have tested the functioning of the human c-myc polyadenylation signal in Saccharomyces cerevisiae. A DNA fragment containing the two AATAAA polyadenylation signals of the c-myc gene was inserted into a plasmid designed for the in-vivo testing of polyadenylation signals in yeast. The c-myc fragment had a partial capacity for directing mRNA 3'-end formation in yeast. The 3'-endpoints were 50-100 bp distant from the mRNA 3'-ends mapped in humans. This human DNA fragment is therefore unspecifically functional in yeast, indicating that other sequence elements than the human polyadenylation signal, AATAAA, are necessary for 3'-end formation.
引用
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页码:201 / 204
页数:4
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