PEROXYOXALATE CHEMILUMINESCENT ASSAY FOR OXIDASE ACTIVITIES BASED ON DETECTING ENZYMATICALLY FORMED HYDROGEN-PEROXIDE

被引：19

作者：

NAKASHIMA, K

KURODA, N

KAWAGUCHI, S

WADA, M

AKIYAMA, S

机构：

[1] School of Pharmaceutical Sciences, Nagasaki University

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1995年 / 10卷 / 03期

关键词：

PEROXYOXALATE CHEMILUMINESCENT ASSAY; OXIDASE; 4,4'-OXALYL-BIS[(TRIFLUOROMETHYLSULPHONYL)IMINO]TRIMETHYLENE-BIS(4-METHYLMORPHOLINIUM)TRIFLUOROMETHANESULPHONATE; 2,4,6,8-TETRAMORPHOLINOPYRIMIDO[5,4-D] PYRIMIDINE;

D O I：

10.1002/bio.1170100307

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive peroxyoxalate chemiluminescent (PO-CL) assay for activities of oxidases (uricase. choline oxidase. cholesterol oxidase and xanthine oxidase) which catalyse a formation of hydrogen peroxide was developed using 4,4'-oxalyl-bis[(trifluoromethylsulphonyl)imino]trimethylene-bis(4-methylmorpholinium)trifluoromethanesulphonate as a chemiluminogenic reagent and 2,4,6,8-tetramorpholinopyrimido[5,4-d]pyrimidine as a fluorophore. The standard curve for hydrogen peroxide was linear over the range 1 x 10(-7)-1 x 10(-4) mol/L. Relative standard deviations for oxidase assays were 5.1-12.7% (n = 10). Detection limits were 1 x 10(-3) U/mL for uricase. 5 x 10(-4) U/mL for choline oxidase. 5 x 10(-3) U/mL for cholesterol oxidase and 5 x 10(-4) U/mL xanthine oxidase (sample to blank ratio, 3).

引用

页码：185 / 191

页数：7

共 16 条

[1] FIBER-OPTIC SENSOR FOR THE DETERMINATION OF GLUCOSE USING MICELLAR ENHANCED CHEMI-LUMINESCENCE OF THE PEROXYOXALATE REACTION [J].