THE ASYMMETRY OF P+ IN BACTERIAL REACTION CENTERS REVEALED BY CIRCULAR-DICHROISM SPECTROSCOPY

被引:6
作者
OLSON, JM [1 ]
MILLER, M [1 ]
DOLIESLAGER, J [1 ]
机构
[1] KATHOLIEKE UNIV LEUVEN,DEPT SCHEIKUNDE,B-3001 HEVERLEE,BELGIUM
关键词
D O I
10.1021/bi00046a031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The circular dichroism anisotropy, (A(L) - A(R))/A, has been measured for the far-red absorption band of P+ in reaction centers of two purple bacteria (Rhodopseudomonas viridis and Rhodobacter sphaeroides) and one green sulfur bacterium (Chlorobium tepidum). The anisotropy values for P960(+) (Rps. viridis) at 1310 nm was found to be +(13 +/- 2) x 10(-4). The corresponding value for P870(+) (Rb. sphaeroides) at 1250 nm was +(11 +/- 1) x 10(-4), but for P840(+) (C. tepidum) at 1160 nm the value was negative: -(27 +/- 2) x 10(-4). These results show that the configuration of the special pair in P840 is significantly different from the configuration in P870 and P960.
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页码:15230 / 15234
页数:5
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