ISOLATION AND SEQUENCE DETERMINATION OF CDNA-ENCODING HUMAN T-PROTEIN OF THE GLYCINE CLEAVAGE SYSTEM

被引：21

作者：

HAYASAKA, K ^{[1
]}

NANAO, K ^{[1
]}

TAKADA, G ^{[1
]}

OKAMURAIKEDA, K ^{[1
]}

MOTOKAWA, Y ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,INST ENZYME RES,TOKUSHIMA 770,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 192卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1480

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the full-length cDNA sequence of human T-protein of the glycine cleavage system. Overlapping cDNA clones were isolated by screening human liver cDNA libraries with bovine T-protein cDNA. The 1209 base pair open reading frame encodes 403 amino acid precursor protein, and the deduced amino acid sequence of the mature peptide shows 90 and 68% homology to that of bovine and chicken counterpart, respectively. RNA blot analysis of human liver transcripts and Southern blot analysis of human genomic DNA confirm that human T-protein is encoded by a single gene. © 1993 Academic Press. All rights reserved.

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页码：766 / 771

页数：6

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