SEX DETERMINATION AND DNA COMPETITION IN THE ANALYSIS OF FORENSIC MIXED STAINS BY PCR

被引：18

作者：

KREIKE, J

LEHNER, A

机构：

[1] Institut für Gerichtliche Medizin, Universität Wien, Wien, A-1090

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1995年 / 107卷 / 05期

关键词：

SEX DETERMINATION; PCR; MIXED STAINS; DNA COMPETITION; SELECTIVE LYSIS;

D O I：

10.1007/BF01245480

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Sex determination of pure and mixed blood samples and stains was performed by amplification of the X-specific and Y-specific alphoid sequences by PCR (XY-PCR). From mixed blood samples with female DNA present in large excess of male DNA, the Y-specific sequence still amplified efficiently. In the analysis of vaginal secretions in a case of sexual assault, XY-PCR was performed to test the efficiency of the selective lysis procedure in order to investigate whether alleles found with other PCR systems were of male or female origin. Our studies with mixed blood samples revealed pronounced DNA competition in the HLA-DQ alpha and D1S80 PCR systems: the alleles from a minor DNA component could not be detected in the presence of a large excess of DNA from a second person.

引用

页码：235 / 238

页数：4

共 13 条

[1] Aasen E., Medrano J.F., Amplification of the ZFX and ZFY genes for sex identification in humans, cattle, sheep and goats, Biotechnology, 8, pp. 1279-1281, (1990)
[2] Gaensslen R.E., Berka K.M., Grosso D.A., Ruano G., Pagliaro E.M., Messina D., Lee H.C., A polymerase chain reaction (PCR) method for sex and species determination with novel controls for deoxyribonucleic acid (DNA) template length, J Forensic Sci, 37, pp. 6-20, (1992)
[3] Gill P., Jeffreys A.J., Werrett D.J., Forensic applications of DNA fingerprints, Nature, 318, pp. 577-579, (1985)
[4] Giusti A., Baird M., Pasquale S., Balasz I., Glassberg J., Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm, J Forensic Sci, 31, pp. 409-417, (1987)
[5] He Z-N, Jiang X-H, Lu S-H, Wang G-L, Zhu Y-W, Wang S-W, Shen Y., Gao Q-S, Liu J-Z, Wu G-Y, A study of sex identification of trace, dried bloodstains using a Y-chromosome-specific deoxyribonucleic acid (DNA) probe, J Forensic Sci, 34, pp. 346-351, (1989)
[6] Mannucci A., Sullivan K.M., Ivanov P.L., Gill P., Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin, Int J Leg Med, 106, pp. 190-193, (1994)
[7] Patri S., Daheron L., Kitzis A., Chomel J-C, Evaluation of bone marrow transplantation efficiency by competitive PCR on Y sequences, PCR Methods Appl., 3, pp. 361-364, (1994)
[8] Pfitzinger H., Ludes B., Mangin P., Sex determination of forensic samples: co-amplification and simultaneous detection of a Y-specific and an X-specific DNA sequence, Int J Leg Med, 105, pp. 213-216, (1993)
[9] Potsch L., Meyer U., Rothschild S., Schneider P.M., Ritmer C., Application of DNA techniques for identification using human dental pulp as a source of DNA, Int J Leg Med, 105, pp. 139-144, (1993)
[10] Potsch L., Prager-Eberle M., Penzes L., Rittner C., HLADQα-Alpha Typisierung an mikroskopisch spermapositiven Ausstrichpräparaten nach PCR, Rechtsmedizin, 3, pp. 54-58, (1993)

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