PURIFICATION AND CHARACTERIZATION OF ALPHA-KETO ESTER REDUCTASES FROM BAKERS-YEAST

被引：43

作者：

NAKAMURA, K ^{[1
]}

KONDO, S ^{[1
]}

KAWAI, Y ^{[1
]}

NAKAJIMA, N ^{[1
]}

OHNO, A ^{[1
]}

机构：

[1] OKAYAMA PREFECTURAL UNIV, FAC HLTH & WELFARE, DEPT NUTR SCI, SOJA, OKAYAMA 71911, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 12期

关键词：

D O I：

10.1271/bbb.58.2236

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three alpha-keto ester reductases (yeast keto ester reductase, YKER-II, -IV, -V) were purified from bakers' yeast. YKER-II, -IV, and -V are dimeric, monomeric, and dimeric enzymes, respectively, and molecular masses are estimated to be 58, 31-39, and 83 kDa, respectively, based on gel filtration and SDS-polyacrylamide electrophoresis. Kinetic parameters and stereoselectivities in reduction of alpha-keto esters have been measured. YKER-IV contributes mainly to reduction by bakers' yeast at low substrate concentrations, and is useful for synthetic purposes.

引用

页码：2236 / 2240

页数：5

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