COMPARATIVE IMMUNOGENICITY OF CONJUGATES COMPOSED OF ESCHERICHIA-COLI O111 O-SPECIFIC POLYSACCHARIDE, PREPARED BY TREATMENT WITH ACETIC-ACID OR HYDRAZINE, BOUND TO TETANUS TOXOID BY 2 SYNTHETIC SCHEMES

被引:30
作者
GUPTA, RK
EGAN, W
BRYLA, DA
ROBBINS, JB
SZU, SC
机构
[1] NICHHD,BETHESDA,MD 20892
[2] US FDA,CTR BIOL EVALUAT & RES,BETHESDA,MD 20892
关键词
D O I
10.1128/IAI.63.8.2805-2810.1995
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Escherichia coli O111, of various H types and virulence factors, causes enteritis throughout the world, especially in young children. This O type is found rarely in healthy individuals. Serum antibodies to the O-specific polysaccharide of O111 lipopolysaccharide (LPS) protect mice and dogs against infection with this E. coli serotype. The O111 O-specific polysaccharide is composed of a pentasaccharide repeat unit with two colitoses bound to the C-3 and C-6 of glucose in a trisaccharide backbone; this structure is identical to that of Salmonella adelaide (O35), another enteric pathogen. Nonpyrogenic O111 O-specific polysaccharide was prepared by treatment of its LPS with acetic acid (O-SP) or the organic base hydrazine (DeA-LPS). The O-SP had a reduced concentration of colitose. These products were derivatized with adipic acid dihydrazide (ADH) or thiolated with N-succinimidyl-3(2-pyridyldithio) propionate (SPDP). The four derivatives were covalently bound to tetanus toroid (TT) by carbodiimide-mediated condensation or with SPDP to form conjugates. Immunization of BALB/c and general-purpose mice by a clinically acceptable route showed that DeA-LPST-TTADH, of the four conjugates, elicited the highest level of LPS antibodies. Possible reasons to explain this differential immunogenicity between the four conjugates are discussed.
引用
收藏
页码:2805 / 2810
页数:6
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