INHIBITION OF DEGRANULATION OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES BY COMPLEMENT-FACTOR-D

被引：31

作者：

BALKE, N

HOLTKAMP, U

HORL, WH

TSCHESCHE, H

机构：

[1] UNIV BIELEFELD, FAC CHEM, DEPT BIOCHEM, D-33501 BIELEFELD, GERMANY

[2] ALLGEMEINES KRANKENHAUS WIEN, NEPHROL & DIALYSIS ABT, VIENNA, AUSTRIA

来源：

FEBS LETTERS | 1995年 / 371卷 / 03期

关键词：

COMPLEMENT FACTOR D; ADIPSIN; RENAL FAILURE; NEUTROPHIL; DEGRANULATION;

D O I：

10.1016/0014-5793(95)00926-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A degranulation inhibiting protein could be isolated from human plasma ultrafiltrate by a three-step purification method including ion-exchange chromatography gelfiltration and affinity-chromatography. The protein was identified as complement factor D by means of sequence analysis, Its degranulation inhibiting activity was determined with regard to its effect on the FNLPNTL-induced lactoferrin secretion of human polymorphonuclear leukocytes. Complement factor D caused a dose-dependent decrease of the FNLPNTL-stimulated lactoferrin degranulation down to 34% of stimulated controls.

引用

页码：300 / 302

页数：3

共 22 条

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