Immortalisation of human urothelial cells

被引：86

作者：

Petzoldt, JL

Leigh, IM

Duffy, PG

Sexton, C

Masters, JRW

机构：

[1] UCL, INST UROL & NEPHROL, LONDON W1P 7PN, ENGLAND

[2] LONDON HOSP, EXPTL DERMATOL LAB, LONDON E1 1BB, ENGLAND

来源：

UROLOGICAL RESEARCH | 1995年 / 23卷 / 06期

关键词：

normal urothelium; in vitro; immortalisation;

D O I：

10.1007/BF00698738

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

A cell line derived from the urothelium lining the ureter of a 12-year-old girl was immortalised using a temperature-sensitive SV40 large T-antigen gene construct, and designated UROtsa. Following immortalisation, UROtsa cells expressed SV40 large T-antigen, but did not acquire characteristics of neoplastic transformation, including growth in soft agar or the development of tumours in nude mice. Metaphase spreads had a normal chromosomal appearance and number. UROtsa cells remained permissive for cell growth at 39 degrees C, indicating that they did not retain temperature sensitivity. UROtsa provides an in vitro model of ''normal'' urothelium.

引用

页码：377 / 380

页数：4

共 11 条

[1]

BOOKLAND EA, 1992, CANCER RES, V52, P1606

[2]

CHRISTIAN BJ, 1987, CANCER RES, V47, P6066

[3] LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS [J].