A MONOCLONAL ANTIPEPTIDE ANTIBODY MIMICS ADRENOCORTICOTROPIC HORMONE-ACTIVITY

被引：4

作者：

CLARKE, BL

BOST, KL

机构：

[1] University of Alabama at Birmingham, Department of Physiology and Biophysics, Birmingham, AL

来源：

IMMUNOLOGY LETTERS | 1991年 / 28卷 / 02期

关键词：

COMPLEMENTARY PEPTIDE; STEROIDOGENESIS; MONOCLONAL ANTIBODY; ACTH RECEPTOR; ADENYLATE CYCLASE; MITOSIS;

D O I：

10.1016/0165-2478(91)90117-S

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A monoclonal antibody that specifically recognizes the adrenocorticotropic receptor (ACTH) on rat adrenal cells was tested for hormonal activity. The antibody behaved as an agonist based on three different biological activities associated with ACTH. An antibody concentration of 16-mu-g/ml stimulated isolated rat adrenal cells to secrete 800 ng/10(4) cells of corticosterone with a concomitant 10-fold increase of cAMP to 30 pmol/10(5) cells. Antibody concentrations above 16-mu-g/ml inhibited mitotic activity in mouse Y-1 adrenal cells. A radio-immunoassay using an anti-ACTH antibody showed that the monoclonal anti-adrenocorticotropic receptor antibody and ACTH are antigenically related. These findings suggest that the anti-receptor antibody recognizes the ligand binding domain of the ACTH receptor.

引用

页码：175 / 180

页数：6

共 12 条

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