ROLE OF A VIRAL MEMBRANE POLYPEPTIDE IN STRAND-SPECIFIC INITIATION OF POLIOVIRUS RNA-SYNTHESIS

被引：95

作者：

GIACHETTI, C ^{[1
]}

SEMLER, BL ^{[1
]}

机构：

[1] UNIV CALIF IRVINE,CALIF COLL MED,DEPT MICROBIOL & MOLEC GENET,IRVINE,CA 92717

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 05期

关键词：

D O I：

10.1128/JVI.65.5.2647-2654.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A molecular genetic analysis has been combined with an in vitro biochemical approach to define the functional interactions required for nucleotidyl protein formation during poliovirus RNA synthesis. A site-directed lesion into the hydrophobic domain of a viral membrane protein produced a mutant virus that is defective in RNA synthesis at 39-degrees-C. The phenotypic expression of this lesion affects initiation of RNA synthesis, in vitro uridylylation of the genome-linked protein (VPg), and the in vivo synthesis of plus-strand viral RNAs. Our results support a model that employs a viral membrane protein as carrier for VPg in the initiation of plus-strand RNA synthesis. Our data also suggest that a separate mechanism could be used in the initiation of minus-strand RNA synthesis, thereby providing a means for strand-specific regulation of picornavirus RNA replication.

引用

页码：2647 / 2654

页数：8

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