STEREOSELECTIVE EFFECT OF WARFARIN AND BILIRUBIN ON THE BINDING OF 5-(ORTHO-CHLOROPHENYL)-1,3-DIHYDRO-3-METHYL-7-NITRO-2H-1,4-BENZODIAZEPIN-2-ONE ENANTIOMERS TO HUMAN SERUM-ALBUMIN

被引：11

作者：

FITOS, I

VISY, J

MAGYAR, A

KAJTAR, J

SIMONYI, M

机构：

[1] HUNGARIAN ACAD SCI, CENT RES INST CHEM, POB 17, H-1525 BUDAPEST, HUNGARY

[2] EOTVOS LORAND UNIV, INST ORGAN CHEM, H-1364 BUDAPEST 5, HUNGARY

来源：

CHIRALITY | 1990年 / 2卷 / 03期

关键词：

affinity chromatography; allosteric interaction; increased enantioselectivity; protein binding; resolution;

D O I：

10.1002/chir.530020307

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The binding of the title benzodiazepine enantiomers and its modulation by warfarin and bilirubin were studied by chromatography on human serum albumin (HSA) immobilized on Sepharose 4B, and also by a combination of ultrafiltration and circular dichroism (UF‐CD) methods. In the absence of warfarin and bilirubin the binding of the benzodiazepine was not stereoselective. (S)‐Benzodiazepine and (S)‐warfarin mutually increased the binding of each other, while the binding of (R)‐benzodiazepine was preferentially enhanced on HSA saturated with bilirubin. Copyright © 1990 Wiley‐Liss, Inc.

引用

页码：161 / 166

页数：6

共 18 条

[1] CIRCULAR-DICHROISM AND GEL-FILTRATION STUDY OF BINDING OF PROCHIRAL AND CHIRAL 1,4-BENZODIAZEPIN-2-ONES TO HUMAN-SERUM ALBUMIN [J].