ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - DIRECT EXPRESSION CLONING AND FUNCTIONAL INTERACTIONS

被引：152

作者：

HESSION, C ^{[1
]}

OSBORN, L ^{[1
]}

GOFF, D ^{[1
]}

CHIROSSO, G ^{[1
]}

VASSALLO, C ^{[1
]}

PASEK, M ^{[1
]}

PITTACK, C ^{[1
]}

TIZARD, R ^{[1
]}

GOELZ, S ^{[1
]}

MCCARTHY, K ^{[1
]}

HOPPLE, S ^{[1
]}

LOBB, R ^{[1
]}

机构：

[1] BIOGEN INC,14 CAMBRIDGE CTR,CAMBRIDGE,MA 02142

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 05期

关键词：

Cell adhesion; Cytokine; Endothelium; Inflammation;

D O I：

10.1073/pnas.87.5.1673

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A cDNA for endothelial leukocyte adhesion molecule 1 (ELAM-1) was isolated by transient expression in COS-7 cells of a subtracted cDNA library from cytokine-treated human umbilical vein endothelial cells (HUVECs), with selection of ELAM-1-expressing clones by adhesion of transfected cells to the human promyelocytic cell line HL-60. This cloning method requires neither antibody nor purified ligand. ELAM-1-expressing COS cells bind the promyelocytic cell line HL-60 by a Ca2+-dependent but temperature-independent mechanism. Although ELAM-1 is homologous to mammalian lectins, its interaction with HL-60 cells is not inhibited by simple carbohydrate structures. ELAM-1-expressing COS cells also bind human neutrophils and the human colon carcinoma cell line HT-29, but not the B-cell line Ramos. However, Ramos cells adhere to cytokine-treated HUVECs but not control HUVECs, confirming the existence of other inducible adhesion molecules. In addition, the binding of HL-60 cells or neutrophils to ELAM-1-expressing COS cells is not inhibited by a monoclonal antibody (60.3) directed to an inhibitory epitope on CD18, indicating that the ELAM-1 ligand, although uncharacterized, is not a member of the CD11/CD18 family.

引用

页码：1673 / 1677

页数：5

共 34 条

[1] INTERACTION OF MURINE GRANULOCYTE MACROPHAGE PROGENITORS AND SUPPORTING STROMA INVOLVES A RECOGNITION - MECHANISM WITH GALACTOSYL AND MANNOSYL SPECIFICITIES [J].