AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF A NEURAMINIDASE INHIBITOR (GG167) IN HUMAN SERUM BY PRECOLUMN FLUORESCENCE DERIVATIZATION USING BENZOIN

被引：13

作者：

STUBBS, RJ

HARKER, AJ

机构：

[1] Drug Metabolism I, Glaxo Research and Development Ltd, Greenford, Middlesex UB6 0HE, Greenford Road

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 670卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00165-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pre-column fluorescence derivatisation high-performance liquid chromatographic method for the analysis of a neuraminidase inhibitor, GG167, in human serum is described. GG167 was extracted from serum samples using Bond Elut SCX solid-phase extraction cartridges, followed by derivatisation with benzoin prior to reversed-phase chromatography with fluorescence detection. This method has been automated using a Zymark robot and used in the analysis of human serum samples from clinical studies. The method has been shown to be valid over a concentration range of 10-800 ng/ml using a 1-ml sample volume.

引用

页码：279 / 285

页数：7

共 6 条

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