MITOGENIC EFFECTS OF PLATELET-DERIVED GROWTH-FACTOR, FIBROBLAST GROWTH-FACTOR, TRANSFORMING GROWTH-FACTOR-BETA, AND HEPARIN-BINDING SERUM FACTOR FOR ADULT-MOUSE SCHWANN-CELLS

被引：40

作者：

WATABE, K ^{[1
]}

FUKUDA, T ^{[1
]}

TANAKA, J ^{[1
]}

TOYOHARA, K ^{[1
]}

SAKAI, O ^{[1
]}

机构：

[1] JIKEI UNIV,SCH MED,DEPT INTERNAL MED 2,TOKYO 105,JAPAN

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1994年 / 39卷 / 05期

关键词：

BROMODEOXYURIDINE; FORSKOLIN; HEPARIN BINDING PROTEIN; S100; TISSUE CULTURE;

D O I：

10.1002/jnr.490390504

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Mitogenic effects of fetal calf serum (FCS), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor-beta (TGF-beta), and forskolin to adult mouse Schwann cells were examined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for S100 and BrdU. PDGF-BB, basic FGF, and TGF-beta 1 and beta 2 were all mitogenic for Schwann cells in media containing FCS, Forskolin suppressed the mitogenic activity of these factors. In serum-free media, PDGF-BB and bFGF were also mitogenic, but TGF-beta 1 and beta 2 were not, Heparin-binding fractions of FCS obtained by heparin-Sepharose chromatography synergized with TGF-beta 1 and beta 2 to produce a mitogenic response, Since PDGF-BB, acidic FGF, and basic FGF were not detected in these fractions by immunoabsorption and immunoblot assays, the presence of unidentified heparin-binding molecules in FCS bioactive for adult mouse Schwann cells is suggested. (C) 1994 Wiley-Liss, Inc.

引用

页码：525 / 534

页数：10

共 39 条

[1] ISOLATION OF ACTIVATED ADULT SCHWANN-CELLS AND A SPONTANEOUSLY IMMORTAL SCHWANN-CELL CLONE [J].

BOLIN, LM ;

IISMAA, TP ;

SHOOTER, EM .

JOURNAL OF NEUROSCIENCE RESEARCH, 1992, 33 (02) :231-238

[2] ESTABLISHMENT AND CHARACTERIZATION OF A MOUSE SCHWANN-CELL LINE WHICH PRODUCES MYELIN INVIVO [J].

BOUTRY, JM ;

HAUW, JJ ;

GANSMULLER, A ;

DIBERT, N ;

POUCHELET, M ;

BARONVANEVERCOOREN, A .

JOURNAL OF NEUROSCIENCE RESEARCH, 1992, 32 (01) :15-26

[3] BIOSYNTHESIS OF MYELIN-ASSOCIATED PROTEINS IN SIMIAN-VIRUS 40 (SV40)-TRANSFORMED RAT SCHWANN-CELL LINES [J].

CHEN, GL ;

HALLIGAN, NLN ;

LUE, NF ;

CHEN, WW .

BRAIN RESEARCH, 1987, 414 (01) :35-48

[4] MITOGENIC RESPONSE OF RAT SCHWANN-CELLS TO FIBROBLAST GROWTH-FACTORS IS POTENTIATED BY INCREASED INTRACELLULAR CYCLIC-AMP LEVELS [J].

CHEN, JK ;

YAO, LL ;

JENQ, CB .

JOURNAL OF NEUROSCIENCE RESEARCH, 1991, 30 (02) :321-327

[5] HEPARIN POTENTIATES THE ACTION OF ACIDIC FIBROBLAST GROWTH-FACTOR BY PROLONGING ITS BIOLOGICAL HALF-LIFE [J].

DAMON, DH ;

LOBB, RR ;

DAMORE, PA ;

WAGNER, JA .

JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 138 (02) :221-226

[6]

DANIELPOUR D, 1990, J BIOL CHEM, V265, P6973

[7] PLATELET-DERIVED GROWTH-FACTORS AND FIBROBLAST GROWTH-FACTORS ARE MITOGENS FOR RAT SCHWANN-CELLS [J].

DAVIS, JB ;

STROOBANT, P .

JOURNAL OF CELL BIOLOGY, 1990, 110 (04) :1353-1360

[8] EVIDENCE THAT THE AXOLEMMAL MITOGEN FOR CULTURED SCHWANN-CELLS IS A POSITIVELY CHARGED, HEPARAN-SULFATE PROTEOGLYCAN-BOUND, HEPARIN-DISPLACEABLE MOLECULE [J].

DECOSTER, MA ;

DEVRIES, GH .

JOURNAL OF NEUROSCIENCE RESEARCH, 1989, 22 (03) :283-288

[9] SCHWANN-CELLS SECRETE A PDGF-LIKE FACTOR - EVIDENCE FOR AN AUTOCRINE GROWTH-MECHANISM INVOLVING PDGF [J].

ECCLESTON, PA ;

COLLARINI, EJ ;

JESSEN, KR ;

MIRSKY, R ;

RICHARDSON, WD .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1990, 2 (11) :985-992

[10] REGULATION OF SCHWANN-CELL PROLIFERATION - MECHANISMS INVOLVED IN PERIPHERAL-NERVE DEVELOPMENT [J].

ECCLESTON, PA .

EXPERIMENTAL CELL RESEARCH, 1992, 199 (01) :1-9

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