TOWARDS A MOLECULAR DESCRIPTION OF PULSED-FIELD GEL-ELECTROPHORESIS

被引：42

作者：

BUSTAMANTE, C ^{[1
]}

GURRIERI, S ^{[1
]}

SMITH, SB ^{[1
]}

机构：

[1] UNIV OREGON,DEPT CHEM,EUGENE,OR 97403

来源：

TRENDS IN BIOTECHNOLOGY | 1993年 / 11卷 / 01期

关键词：

D O I：

10.1016/0167-7799(93)90071-G

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An improved understanding of the molecular processes of migration and reorientation of DNA molecules undergoing conventional and pulsed-field gel electrophoresis (PFGE) should provide the basis for the rational optimization of these techniques. This review of common pulsed-field methods emphasizes the range of their applications as well as their current limitations. The molecular mechanisms involved in electrophoresis, as they are beginning to emerge from a combination of direct observations using a microscope and computer-modeling studies, are also discussed.

引用

页码：23 / 30

页数：8

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