PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF ALPHA-TOXIN OF CLOSTRIDIUM-PERFRINGENS

被引：17

作者：

BASAK, AK

STUART, DI

NIKURA, T

BISHOP, DHL

KELLY, DC

FEARN, A

TITBALL, RW

机构：

[1] NERC,INST VIROL,OXFORD OX2 6SR,ENGLAND

[2] OXFORD CTR MOLEC SCI,NEW CHEM LAB,OXFORD OX1 3RE,ENGLAND

[3] CHEM & BIOL DEF ESTAB,SALISBURY SP4 0JQ,WILTS,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 244卷 / 05期

关键词：

TOXIN; X-RAY DIFFRACTION; PURIFICATION; CRYSTALLIZATION; STRUCTURE;

D O I：

10.1006/jmbi.1994.1758

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alpha-toxin of Clostridium perfringes, cloned in Escherichia coli, has been purified and crystallized from ammonium sulphate using the hanging drop vapour diffusion method at 20 degrees C. The crystals diffract to a minimum Bragg spacing of 2.7 Angstrom, belong to the space group R32 (with a = b = 153.3 Angstrom, c = 95.4 Angstrom, alpha = beta = 90 degrees and gamma = 120 degrees) and contain a single polypeptide chain in the crystallographic unit.

引用

页码：648 / 650

页数：3

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