DEVELOPMENT AND APPLICATION OF A NEW ENZYME SENSOR TYPE BASED ON THE EIS-CAPACITANCE STRUCTURE FOR BIOPROCESS CONTROL

被引：41

作者：

BEYER, M ^{[1
]}

MENZEL, C ^{[1
]}

QUACK, R ^{[1
]}

SCHEPER, T ^{[1
]}

SCHUGERL, K ^{[1
]}

TREICHEL, W ^{[1
]}

VOIGT, H ^{[1
]}

ULLRICH, M ^{[1
]}

FERRETTI, R ^{[1
]}

机构：

[1] UNIV HANNOVER,INST HALBLEITERTECHNOL & WERKSTOFFE ELEKTROTECH,W-3000 HANNOVER,GERMANY

来源：

BIOSENSORS & BIOELECTRONICS | 1994年 / 9卷 / 01期

关键词：

ENZYME SENSOR; EIS-STRUCTURES; C/V MEASUREMENTS; FIA-SYSTEM;

D O I：

10.1016/0956-5663(94)80010-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

New biosensors were developed by immobilizing enzymes on simple EIS (Electrolyte Insulator Semiconductor) layer structures. These EIS-structures produced by means of semiconductor technologies are either pH-(Si/SiO2/Si3N4/Ta2O5 layers) or pF-sensitive (Si/SiO2/Si3N4/LaF3 layers). A schematic of the layered structure is shown in Fig. 1. The basic principle for measurements with the modified EIS-layer structures is the change in capacitance in dependence on the concentration of H+ or F- ions. Measurements were made by using a C/V-measuring instrument in a ''constant charge mode''. These biosensors as well as a reference electrode were integrated into an FIA-system (Flow Injection Analyser) and the concentrations of penicillin and urea (with pH-sensor) and H2O2 and glucose (with pF-sensor) were determined.

引用

页码：17 / 21

页数：5

共 3 条

[1] INTEGRATED DIFFERENTIAL ENZYME SENSORS USING HYDROGEN AND FLUORIDE-ION SENSITIVE MULTIGATE FETS [J].

HINTSCHE, R ;

DRANSFELD, I ;

SCHELLER, F ;

PHAM, MT ;

HOFFMANN, W ;

HUELLER, J ;

MORITZ, W .

BIOSENSORS & BIOELECTRONICS, 1990, 5 (04) :327-334

[2]

HO MH, 1988, SENSOR ACTUATOR, V15, P446

[3] FLUORIDE-SENSITIVE MEMBRANE FOR ISFETS [J].

MORITZ, W ;

MEIERHOFER, I ;

MULLER, L .

SENSORS AND ACTUATORS, 1988, 15 (03) :211-219

← 1 →