GROWTH-PARAMETERS, PROTEIN AND DNA-SYNTHESIS OF AN EMBRYOGENIC SUSPENSION-CULTURE OF WHITE SPRUCE (PICEA-GLAUCA)

被引：8

作者：

DONG, JZ ^{[1
]}

DUNSTAN, DI ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA, INST PLANT BIOTECHNOL, SASKATOON S7N 0W9, SK, CANADA

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1994年 / 144卷 / 02期

关键词：

DNA; EMBRYOGENIC SUSPENSION CULTURE; PROTEIN; GROWTH PARAMETERS; PICEA GLAUCA;

D O I：

10.1016/S0176-1617(11)80544-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Growth parameters of a white spruce (Picea glauca) embryogenic suspension culture were characterized. Tissue fresh mass, dry mass, number of immature somatic embryos (stage 1), intracellular protein content, and total DNA accumulation showed similar growth curves over a 21-d culture period. Extracellular proteins gradually accumulated during the first 9 d, then dramatically increased. Immature embryo number showed a positive correlation to intracellular protein and DNA accumulations, correlation coefficients for these parameters were higher than for fresh and dry masses. The results from in vivo pulse-labelling with S-35-methionine showed a peak for incorporation into proteins at day 7. The protein profiles of intracellular proteins were greatly different from those of extracellular proteins.

引用

页码：201 / 208

页数：8

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