OPEN-TUBULAR LIQUID-CHROMATOGRAPHY WITH SURFACTANT-ENHANCED ELECTROOSMOTIC FLOW

被引：60

作者：

PFEFFER, WD

YEUNG, ES

机构：

[1] US DOE,AMES LAB,AMES,IA 50011

[2] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 20期

关键词：

D O I：

10.1021/ac00219a005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The velocity of electroosmotic flow in a polymer-coated, reversed-phase fused silica column is enhanced by low concentrations (μM) of cetyltrimethylammonium bromide (CTAB) In the buffer. Open-tubular capillary liquid chromatography (OTCLC) based upon this enhanced flow makes possible the separation of several neutral, geometrical, and positional Isomers with greater than baseline resolution (R > 1) in all cases and efficiencies of up to 230 000 plates generated In less than 4 min In a 54-cm column. As little as 5-pL samples can be directly Injected, and as low as 0.75 amol of 9-methylanthracene can be detected. This brings to OTCLC the instrumental simplicity of capillary electrophoresis. © 1990, American Chemical Society. All rights reserved.

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页码：2178 / 2182

页数：5

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