BROADLY NEUTRALIZING ANTIBODIES ELICITED BY THE HYPERVARIABLE NEUTRALIZING DETERMINANT OF HIV-1

被引：371

作者：

JAVAHERIAN, K ^{[1
]}

LANGLOIS, AJ ^{[1
]}

LAROSA, GJ ^{[1
]}

PROFY, AT ^{[1
]}

BOLOGNESI, DP ^{[1
]}

HERLIHY, WC ^{[1
]}

PUTNEY, SD ^{[1
]}

MATTHEWS, TJ ^{[1
]}

机构：

[1] DUKE UNIV,SCH MED,DEPT SURG,DURHAM,NC 27710

来源：

SCIENCE | 1990年 / 250卷 / 4987期

关键词：

D O I：

10.1126/science.1703322

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The principal neutralizing determinant (PND) of human immunodeficiency virus (HIV)-1 resides within the V3 loop of the envelope protein. Antibodies elicited by peptides of this region were able to neutralize diverse isolates. Serum from one of three animals immunized with the human T cell lymphoma virus (HTLV)-IIIMN PND peptide, RP142, neutralized MN and the sequence-divergent HTLV-IIIB isolate. Serum from one of three animals immunized with a 13-amino acid IIIB PND peptide (RP337) also neutralized both of these isolates. Characterization of these sera revealed that the cross-neutralizing antibodies bound the amino acid sequence GlyProGlyArgAlaPhe (GPGRAF) that is present in both isolates. This sequence is frequently found in the PNDs analyzed in randomly selected HIV-1 isolates. Sera from two rabbits immunized with a peptide containing only the GPGRAF residues neutralized divergent isolates, including IIIB and MN.

引用

页码：1590 / 1593

页数：4

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EMINI E, UNPUB

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