INFLUENCE OF IONIC STRENGTH ON ACTIVITY OF TRYPSIN AND ACETYLTRYPSIN

被引：15

作者：

BECHET, JJ

CHEVALLIER, J

LABOUESSE, J

ARNOULD, B

ALDINCIC.S

YON, J

机构：

[1] Laboratoire d'Enzymologie Chimique, Institut de Biochimie, Laboratoire d'Enzymologie Physico-Chimique et Moléculaire, Orsay

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 151卷 / 01期

关键词：

D O I：

10.1016/0005-2744(68)90171-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The action of ionic strength on the esterasic activity of trypsin (EC 3.4.4.4) has been investigated with specific substrates. A substantial effect of ionic strength on the ionisation pK of the histidine located at the active center is observed. The shift of this pK is explained by a repulsive interaction between the charged imidazole group and two positive charges. Some comparative experiments carried out with acetyltrypsin led to the identification of these charges as the ε-NH3+ of two lysine residues. © 1968.

引用

页码：165 / +

页数：1

共 27 条

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