IDENTIFICATION OF CLEAVAGE SITES INVOLVED IN PROTEOLYTIC PROCESSING OF PSEUDOMONAS-AERUGINOSA PREPROELASTASE

被引：26

作者：

KESSLER, E ^{[1
]}

SAFRIN, M ^{[1
]}

PERETZ, M ^{[1
]}

BURSTEIN, Y ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT ORGAN CHEM,IL-76100 REHOVOT,ISRAEL

来源：

FEBS LETTERS | 1992年 / 299卷 / 03期

关键词：

PROTEOLYTIC PROCESSING; PREPROELASTASE PROCESSING; ELASTASE; PSEUDOMONAS-AERUGINOSA;

D O I：

10.1016/0014-5793(92)80134-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extracellular elastase (33 kDa) of Pseudomonas aeruginosa is synthesized as a 53.6 kDa preproenzyme containing a long, N-terminal propeptide. The free propeptide and the elastase precursor generated upon propeptide removal were isolated from P. aeruginosa cells and subjected to N-terminal amino acid sequence analysis. The results identified Ala-174 and Ala+1 as the amino terminal residues of the propeptide and the elastase precursor, respectively, indicating that: (1) the signal peptide consists of 23 amino acid residues and its molecular weight is 2.4 kDa, (2) the propeptide contains 174 amino acid residues and is of 18.1 kDa molecular weight, and (3) no additional N-terminal proteolytic cleavage is required for elastase maturation.

引用

页码：291 / 293

页数：3

共 14 条

[1] MOLECULAR CHARACTERIZATION AND NUCLEOTIDE-SEQUENCE OF THE PSEUDOMONAS-AERUGINOSA ELASTASE STRUCTURAL GENE [J].

BEVER, RA ;

IGLEWSKI, BH .

JOURNAL OF BACTERIOLOGY, 1988, 170 (09) :4309-4314

[2] MECHANISMS OF ACTIVATION AND SECRETION OF A CELL-ASSOCIATED PRECURSOR OF AN EXOCELLULAR PROTEASE OF PSEUDOMONAS-AERUGINOSA 34362A [J].

FECYCZ, IT ;

CAMPBELL, JN .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 146 (01) :35-42

[3] STRUCTURAL GENE AND COMPLETE AMINO-ACID SEQUENCE OF PSEUDOMONAS-AERUGINOSA IFO-3455 ELASTASE [J].

FUKUSHIMA, J ;

YAMAMOTO, S ;

MORIHARA, K ;

ATSUMI, Y ;

TAKEUCHI, H ;

KAWAMOTO, S ;

OKUDA, K .

JOURNAL OF BACTERIOLOGY, 1989, 171 (03) :1698-1704

[4]

HUNKAPILLER MW, 1983, METHOD ENZYMOL, V91, P227

[5]

IGLEWSKI BH, 1990, PSEUDOMONAS : BIOTRANSFORMATIONS, PATHOGENESIS, AND EVOLVING BIOTECHNOLOGY, P36

[6] SYNTHESIS, PROCESSING, AND TRANSPORT OF PSEUDOMONAS-AERUGINOSA ELASTASE [J].

KESSLER, E ;

SAFRIN, M .

JOURNAL OF BACTERIOLOGY, 1988, 170 (11) :5241-5247

[7] PARTIAL-PURIFICATION AND CHARACTERIZATION OF AN INACTIVE PRECURSOR OF PSEUDOMONAS-AERUGINOSA ELASTASE [J].

KESSLER, E ;

SAFRIN, M .

JOURNAL OF BACTERIOLOGY, 1988, 170 (03) :1215-1219

[8]

KESSLER E, 1986, COLLAGEN REL RES, V6, P249

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] SUBSTITUTION OF ACTIVE-SITE HIS-223 IN PSEUDOMONAS-AERUGINOSA ELASTASE AND EXPRESSION OF THE MUTATED LASB ALLELES IN ESCHERICHIA-COLI SHOW EVIDENCE FOR AUTOPROTEOLYTIC PROCESSING OF PROELASTASE [J].

MCIVER, K ;

KESSLER, E ;

OHMAN, DE .

JOURNAL OF BACTERIOLOGY, 1991, 173 (24) :7781-7789

← 1 2 →