A BIOASSAY FOR CHOLERA-LIKE TOXINS USING HT29 CELLS

被引：5

作者：

CHARANIA, Z ^{[1
]}

VANMAELE, R ^{[1
]}

ARMSTRONG, GD ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT MED MICROBIOL & INFECT DIS,EDMONTON T6G 2H7,ALBERTA,CANADA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1991年 / 14卷 / 03期

关键词：

CHOLERA TOXIN; ESCHERICHIA-COLI; HEAT-LABILE TOXIN; HT29; CELL;

D O I：

10.1016/0167-7012(91)90020-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this article we describe an alternative to the Chinese hamster ovary (CHO) cell elongation assay for detecting cholera-like toxins in biological samples. The assay is based on the observation that cholera toxin (CT) and heat-labile toxin (LT) from Escherichia coli cause the formation of large vacuoles in human colonic adenocarcinoma HT29 cells. The response time of HT29 cells to CT or LT was the same as that of CHO cells but much lower concentrations of CT activity could be detected with the HT29 cell assay. Vacuole formation appeared to be dependent on the presence of the ADP-ribosyltransferase subunit of CT.

引用

页码：171 / 176

页数：6

共 13 条

[1]

Altman DG, 1991, PRACTICAL STAT MED R, P179

[2]

BOKOCH GM, 1983, J BIOL CHEM, V258, P2072

[3] ROLE OF THE A-SUBUNIT OF PERTUSSIS TOXIN IN ALTERATION OF CHINESE-HAMSTER OVARY CELL MORPHOLOGY [J].

BURNS, DL ;

KENIMER, JG ;

MANCLARK, CR .

INFECTION AND IMMUNITY, 1987, 55 (01) :24-28

[4] ADHESION OF ENTEROTOXIGENIC ESCHERICHIA-COLI TO THE HUMAN COLON-CARCINOMA CELL-LINE CACO-2 IN CULTURE [J].

DARFEUILLE-MICHAUD, A ;

AUBEL, D ;

CHAUVIERE, G ;

RICH, C ;

BOURGES, M ;

SERVIN, A ;

JOLY, B .

INFECTION AND IMMUNITY, 1990, 58 (04) :893-902

[5] INVASION AND REPLICATION OF SALMONELLA-TYPHIMURIUM IN ANIMAL-CELLS [J].

GAHRING, LC ;

HEFFRON, F ;

FINLAY, BB ;

FALKOW, S .

INFECTION AND IMMUNITY, 1990, 58 (02) :443-448

[6] INDUCTION OF A NOVEL MORPHOLOGICAL RESPONSE IN CHINESE-HAMSTER OVARY CELLS BY PERTUSSIS TOXIN [J].

HEWLETT, EL ;

SAUER, KT ;

MYERS, GA ;

COWELL, JL ;

GUERRANT, RL .

INFECTION AND IMMUNITY, 1983, 40 (03) :1198-1203

[7] SENSITIVE METHOD FOR DETECTING LOW NUMBERS OF VEROTOXIN-PRODUCING ESCHERICHIA-COLI IN MIXED CULTURES BY USE OF COLONY SWEEPS AND POLYMYXIN EXTRACTION OF VEROTOXIN [J].

KARMALI, MA ;

PETRIC, M ;

LIM, C ;

CHEUNG, R ;

ARBUS, GS .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (04) :614-619

[8] VERO RESPONSE TO A CYTOTOXIN OF ESCHERICHIA-COLI [J].

KONOWALCHUK, J ;

SPEIRS, JI ;

STAVRIC, S .

INFECTION AND IMMUNITY, 1977, 18 (03) :775-779

[9] ADHESION OF COLONIZATION FACTOR ANTIGEN-II-POSITIVE ENTERO-TOXIGENIC ESCHERICHIA-COLI STRAINS TO HUMAN ENTEROCYTE-LIKE DIFFERENTIATED HT-29 CELLS - A BASIS FOR HOST-PATHOGEN INTERACTIONS IN THE GUT [J].

NEESER, JR ;

CHAMBAZ, A ;

GOLLIARD, M ;

LINKAMSTER, H ;

FRYDER, V ;

KOLODZIEJCZYK, E .

INFECTION AND IMMUNITY, 1989, 57 (12) :3727-3734

[10] IDENTIFICATION OF A HUMAN-ERYTHROCYTE RECEPTOR FOR COLONIZATION FACTOR ANTIGEN-I PILI EXPRESSED BY H10407 ENTERO-TOXIGENIC ESCHERICHIA-COLI [J].

PIERONI, P ;

WOROBEC, EA ;

PARANCHYCH, W ;

ARMSTRONG, GD .

INFECTION AND IMMUNITY, 1988, 56 (05) :1334-1340

← 1 2 →